10/29/2022 0 Comments Stain precipitate![]() ![]() ![]() Platelet granules are a pink-purple, more regularly spaced, and more variable in size versus Mycoplasma wenyonii organisms.īrandy Kastl, DVM, DACVP is a clinical pathologist in the KSVDL. At times, the granules in ruminant platelets (black arrow) can mimic these organisms, particularly when platelets are clumped or pale-staining. The organisms are small (<0.5um) pale lavender to grey and present in cocci, ring and rod-shaped forms. Very few Mycoplasma wenyonii organisms adhered to red blood cells is a common finding. ![]() #STAIN PRECIPITATE FREE#Large numbers of wenyonii(green arrow) are seen free in the background of the smear. These structures tend to stain blue rather than purple, are more regularly round versus Anaplasma marginale, and usually within the erythrocyte rather than along the margin of the cells.ĥ. Image B is of a Howell-Jolly body, which are nonpathogenic erythrocyte nuclear remnants. The green arrow in image A denotes water artifact along the margin of the erythrocyte which can sometimes mimic Anaplasma marginale See answer to question 3 for information on water artifacts. organisms, Anaplasma marginale is a bit lager and denser. Anaplasma marginale appear as prominent, deep purple structures along the erythrocyte margin. A is the image of Anaplasma marginale organisms in bovine erythrocytes (black arrows). Using a hairdryer on the cool or low setting can aid in rapidly drying blood smears and eliminating pesky water artifact.Ĥ. Allowing slides to dry completely prior to staining can eliminate this artifact. When the fine focus is slightly adjusted on this artifact it will change from a dark color to clear, bright, and refractile as the light from the microscope bends through the water droplets. It can also line the peripheral margin of the cells mimicking either Anaplasma or Mycoplasma spp. This artifact can be of any shape and on any location in the red cell. Image B shows water artifact on the erythrocytes (blue arrows). Notice the classic teardrop and rounded shape with a peripheralized dark, crescent-shaped nucleus. A is the image of Cytauxzoon felis piroplasms inside feline erythrocytes. Regularly changing rapid stains at least once monthly or more often as needed can reduce this artifact.ģ. Notice the irregularity and variation in shape and location on the erythrocytes. Image A shows stain precipitate on the erythrocytes and unevenly distributed throughout the slide background (blue arrows). Notice the consistent size, placement, and staining intensity of the Mycoplasma organisms along the peripheral margin of the erythrocytes (black arrows). B is the image of hemotropic Mycoplasma organisms on the surface of a feline erythrocyte. Notice the regular distribution of the platelet granules and the variation in platelet size.Ģ. Image A shows multiple normal canine platelets (blue arrows) superimposed over canine erythrocytes. These organisms are round, tear drop, or sometimes ameboid shaped with a single nucleus often to the peripheral margin of the organism (black arrow). B is the image of Babesia canis piroplasms inside a canine erythrocyte. Which arrow below depicts Mycoplasma wenyonii organisms in a bovine blood film (100x oil objective)?ġ. Which picture below depicts Anaplasma marginale organisms on bovine red blood cells (100x oil objective)?ĥ. Which picture below depicts Cytuaxzoan felis piroplasms in feline red blood cells (100x oil objective)?Ĥ. Which picture below depicts hemotropic Mycoplasma on feline red blood cells (100x oil objective)?ģ. Which image below depicts Babesia canis piroplasms inside a canine red blood cell (100x oil objective)?Ģ. (Answers at the bottom of the page!) All images are stained with Modified-Wright stain.ġ. The following quiz is to test your knowledge differentiating hemoparasites from common artifacts and provide tips and tricks for differentiating these entities. However, numerous artifacts commonly seen on blood smears can mimic hemoparasites. Blood film examination is a rapid and cost-effective method to evaluate for hemoparasites. Hemoparasites are a differential diagnosis for animals with anemia. October 2020 FAKE OUT! Hemoparasite or Blood Smear Artifact ![]()
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